General Information
    • ISSN: 1793-821X (Print)
    • Abbreviated Title: J. Clean Energy Technol.
    • Frequency: Quarterly
    • DOI: 10.18178/JOCET
    • Editor-in-Chief: Prof. Haider F. Abdul Amir
    • Executive Editor: Ms. Jennifer Zeng
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Universiti Malaysia Sabah, Malaysia.
I would like to express my appreciation to all the reviewers and editors, who have been working
very hard to ensure the quality of the journal. It's my honor to work with such a wonderful team.

JOCET 2013 Vol. 1(3): 189-193 ISSN: 1793-821X
DOI: 10.7763/JOCET.2013.V1.43

Steady State Performance of a Bioreactor for Production of near Zero Sulfur Diesel (NZSD) and Bio-surfactant

Sujaya Bandyopadhyay, Ranjana Chowdhury, and Chiranjib Bhattacharjee
Abstract—Kinetics of biodesulfurization of hydrotreated diesel using Rhodococcus sp has been studied with special reference to removal of organo-sulfur compounds in diesel and production of 2-hydroxy biphenyl. The identification of 2-HBP in treated diesel has been made using HPLC,FTIR and GCMS .The sulfur concentration of feed diesel was in the range of 200-540 mg/L. Aqueous phase to diesel ratios were varied in the range of 9:1 to1:9. The optimum ratio was found to be 1:4 and the maximum conversion of sulfur was determined to be 95%. The values of Monod kinetic parameters namely, μ max, maximum specific growth rate and Ks, saturation constant of the microbial growth and Yield coefficient of surfactant have been measured to be 0.096h-1 , 71mg/L , and 17 μmol/g dry cell weights respectively by conducting batch type experiments. A continuous chemostat was studied using different hydrodynamic and physico-chemical parameters like dilution rate, initial concentration of organo-sulfur compounds in diesel, stirring rate and aeration rate. Surfactant part was characterized by determination of surface tension (Ring method), E24, TLC, HPLC and GC-MS. The interfacial tension of the supernatant fermented by Rhodococcus sp decreased from 28 dynes/cm to 9 dynes/cm.The surface tension of aqueous nutrient phase and diesel was observed to decrease from 71 dynes/cm to 30 dynes/cm and to 20 dynes/cm from 30dynes/cm respectively. Values of emulsification index (E24) were determined to vary from 18 to 58 over the growth period of 2 to 48 hours in the chemostat. The critical miceller concentration was found to be 200mg/L. The simulated data have been compared with the experimental ones to validate the model.

Index Terms—Biodesulfuirzation of diesel, biosurfactant, emulsification index, mathematical model, Monod kinetics steady state, surface Tension

The authors are with Chemical Engineering Department Jadavpur University, Kolkata, India (e-mail:;;


Cite:Sujaya Bandyopadhyay, Ranjana Chowdhury, and Chiranjib Bhattacharjee, "Steady State Performance of a Bioreactor for Production of near Zero Sulfur Diesel (NZSD) and Bio-surfactant," Journal of Clean Energy Technologies vol. 1, no. 3, pp. 189-193, 2013.

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