Abstract—The oleaginous microalgae and yeasts have been suggested as a promising biodiesel feedstock because of their potential for lipid production under various mode of cultivation. In this study, microbial biomass and lipid production to use as biodiesel feedstock by microalgae Chlorella sp. KKU-S2 and yeast TorulasporaglobosaYU5/2 via integrated cultivation technique using CO2 emissions from yeast cultivation as inorganic carbon source for mixotrophic growth of microalgae were investigated. A maximum specific growth rate of Chlorella sp. KKU-S2 of 0.380(1/d) was obtained via integrated growth using CO2 emissions from yeast T.globosaYU5/2 grown on molasses while specific growth rate of 0.219(1/d) was found via non-integrated growth. A high value of lipid productivity (QP, 0.338 g/L/d), specific product yield (YP/X, 0.202), biomass productivity (QX, 1.633g/L/d) of Chlorella sp. KKU-S2 were found by integrated growth with T.globosaYU5/2 cultivation on molasses, overall biomass and lipid yield of 17.71g/L and 2.89g/L was obtained, respectively. To our knowledge there are a few reports about the microbial biomass and microbial lipid production from isolated microalgae Chlorella sp. KKU-S2 and isolated yeast T.globosaYU5/2in an integrated technique by using CO2 emissions from yeast cultivation.
Index Terms—Microbial lipid, biodiesel feedstock, microalgae, oleaginous yeast, integrated cultivation.
Rattanaporn Baojungharn, Mutiyaporn Paungbut, and Supaporn Kookkhunthodare are with the Graduate School of Khon Kaen University, Khon Kaen 40002, Thailand (e-mail: birdjidrid@windowslive.com, mutiyapornp@kkumail.com, supaporn.kookkhunthod.mam@gmail.com).
Ratanaporn Leesing is with the Department of Microbiology, Faculty of Science, Alternative Energy Research and Development (AERD) and Fermentation Research Center for Value Added Agricultural Products (FerVAAP), Khon Kaen University, Khon Kaen 40002, Thailand (e-mail: ratlee@kku.ac.th).
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Cite:Rattanaporn Baojungharn, Mutiyaporn Paungbut, Supaporn Kookkhunthod, and Ratanaporn Leesing, "Production of Biodiesel Feedstock by Integrated Growth of Isolated Oleaginous Yeast and Microalgae," Journal of Clean Energy Technologies vol. 4, no. 4, pp. 267-270, 2016.
